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Sartorius Vivacon 500 100.000 MWCO HYDROS. 100/BOX - 163X130X140

Sartorius Vivacon 500 100.000 MWCO HYDROS. 100/BOX - 163X130X140

467,00 € *
Inhalt: 100 Stück (4,67 € * / 1 Stück)

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  • 1919101
  • VN01H42
Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of... mehr

Vivacon® 500 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 500 µl initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 500 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable human genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 500 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP1). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations. 1Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62. Concentration of up to 0.5 ml genomic DNA or peptide samples Low binding material Reverse spin possibility MWCO printed on device Complete and highly reproducible concentrate recovery Selected cut offs available sterile and DNA free* for e.g. forensic applications * qPCR assay over 55 cycles with a verified limit of detection < 2 pg Performance Characteristics for DNA Start volume 0.5 ml, sample concentration 50 ng/ml.

Sample size (bp) Time to concentrate up to 30x [min.] at 20°C Concentrate recovery % g-force (xg)
2,000 MWCO 10 60 min. 93 % 7,500
10,000 MWCO 30 25 min. 94 % 7,500
30,000 MWCO 50 18 min. 88 % 5,000
50,000 MWCO 300 18 min. 91 % 5,000
100,000 MWCO 600 10 min. 87 % 3,000
125,000 MWCO 650 12 min. 85 % 2,000
125,000 MWCO 900 9 min. 94 % 3,000
Performance Characteristics for Proteins Start volume 0.5 ml, sample and concentration of proteins as specified in table.
Sample Time to concentrate up to 30x [min.] at 20°C Concentrate recovery % g-force (xg)
2,000 MWCO 0.25 mg/ml cytochrome c 30 min. 95 % 14,000
10,000 MWCO 0.25 mg/ml cytochrome c 15 min. 92 % 14,000
30,000 MWCO 1.0 mg/ml BSA 10 min. 95 % 14,000
50,000 MWCO 1.0 mg/ml BSA 10 min. 92 % 14,000
100,000 MWCO 1.0 mg/ml bovine IgG 11 min. 90 % 8,000
125,000 MWCO 1.0 mg/ml bovine IgG 10 min. 81 % 8,000

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Sartorius

Die Kunden von Sartorius arbeiten hart daran, Medikamente zu entwickeln und herzustellen, die das Fortschreiten von Krankheiten heilen, verhindern oder stoppen. Um erfolgreich zu sein, verlassen sie sich auf Partner, die ihre aktuellen und zukünftigen Herausforderungen verstehen. Seit 150 Jahren steht die Marke Sartorius für innovative Produkte und Lösungen, die die Arbeit von Wissenschaftlern und Ingenieuren weltweit unterstützen. Dabei hat Satorius sich zunehmend darauf konzentriert, Durchbrüche in der Life-Science-Forschung und der Bioproduktion zu ermöglichen.

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